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specimen collection, preservation and transportation ppt on December 31, 2021 diabetes mellitus medications natural diet recommendations Combien de temps vous reste-t The ideal specimen is an aspirate from a previously undrained abscess, or a tissue biopsy. 29 Mai . There is a clot activator present and inversion expedites the clotting process. - Prefilled Specimen Containers is a convenient method for both physicians and medical professionals to collect transport and preserve blood, platelets and plasma for long-term study or research. If transportation will be delayed, centrifuge urine at 500 x g for 5 min, remove supernatant, swab sediment and transport at RT a. What specimens are to be collected? endstream endobj 367 0 obj <>stream Click here to review the details. Role of specimen collection in infectious disease by Dr. T. V. Rao MD . Proper preservation ensures a high quality specimen, which increases the quality of information the specimen contains, and increases the value of the maintenance of the specimen. COLLECTION AND TRANSPORT Specimen Collection Clinically Relevant Microbiology Starts at the Source, - Specimen Collection Clinically Relevant Microbiology Starts at the Source Mike Costello, PhD, MT(ASCP) ACL Laboratories 847.349.7403 mike.costello@advocatehealth.com. Midstream "Clean-Catch" Urine Specimen 4.3. Tap here to review the details. Use the container/tube indicated in the test requirements for appropriate specimen preservation. All specimen containers must be transported in a double-sided, self-sealing polythene bag with one compartment containing the laboratory request form and the other the specimen. endobj EDTA : A calcium chelating agent; can yield false magnesium and calcium results if drawn before a red top tube. Oxalate : Interferes with cell membranes, Reasons for a Rejected Specimen No Test Requisition Form (TRF) Test not written on TRF Submitted wrong specimen for test requested Quantity Not Sufficient (QNS) Missing or inadequate identiification Clotted, Avoiding Common Errors in Specimen Collection Insufficient quantity Hemolysis Failure to use the correct container for specimen collection Inaccurate and incomplete patient guidance Failure to label a specimen correctly and to provide all pertinent information Failure to tighten specimen container lids, resulting in leakage and/or contamination of specimen, To prevent a hematoma: Puncture only the uppermost wall of the vein Make sure the needle fully penetrates the upper most wall of the vein. endstream endobj startxref Do you have PowerPoint slides to share? Expired tubes with low vacuum (rare). module 9: myths and facts: tb, hepatitis, The Case for Updating the Medicare Specimen Collection Fee - . Completion of the appropriate specimen submission form. Collect 5-10 ml of venous blood in a dry sterile, screw-cup glass tube or bottle. Skin specimens are required to investigate infections caused by herpes simplex viruses, rabies virus. Date, time and initials of the phlebotomist must be on the label of EACH tube. Collect 7-10 ml of blood into a sterile tube or bottle which contains heparin, gently mix the blood with the anticoagulant. hb```WB cbC Unlabeled specimen(s) or incorrectly labeled specimen(s) All blood specimens that are mislabeled or unlabeled will be redrawn. Once a sample has been collected, the device should be sealed according to the manufacturer's instructions. For body fluid collection provide the patient with the suitable container and collection instruction . The UPMC clinical virology lab requires specimens for virus culture be collected in the VTM 'M4'. Full clinical information: nature duration, and severity of the disease. A specimen of nasopharyngeal secretion can also be obtained by using a sterile mucus extractor. EQUIPMENTcon., Alcohol Wipes - 70% isopropyl alcohol. What is the collection order of tubes? Urine specimen stability under shipping conditions other than those recommended has not been evaluated. Activate your 30 day free trialto continue reading. Do not sell or share my personal information, 1. Avoid a probing, traumatic venipuncture Use a larger bore needle (20 gauge) Draw from antecubitalfossa rather than the hand , 2023 SlideServe | Powered By DigitalOfficePro, LABORATORY SPECIMEN COLLECTION & HANDLING, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. Conducting regular self-management tasks such as medication and insulin intake, blood sugar checkup, diet observance, and physical exercise are really demanding. Closed system collection - collect from the catheter tubing. role of the nurse. Check the requisition form for requested tests, patient information, and any special requirements. Scheduled Hours: 40. NE P S OR SP CIM N L CT ANDLING OF PowerShow.com is brought to you byCrystalGraphics, the award-winning developer and market-leading publisher of rich-media enhancement products for presentations. Postural effects are accentuated in patients with edema and low plasma protein values. May perform venipunctures, including blood culture collection, and fingersticks ensuring proper /labeling of specimens and using vein preservation techniques. After few seconds, slowly withdraw the swab and immerse the swab in a container of sterile VTM, cutting off swab wire to allow the bottle cap to be replaced tightly. <> Urine collection. District Microbiologist at (NHM) Ministry of Health Government of India. Normal reference ranges are often noted according to age. What is the amount of specimen necessary for testing? JITENDRA KUMAR PANDEY The essential elements of the requisition cont., Histopathology specimen request must contain in addition to the above listed information, the following: name of the procedure, no. Sputum Specimen and Culture 3. 5 0 obj Concepts of Specimen Collection Importance of Specimen Collection Nurses' Roles in Specimen Collection 1. module 2: specimen collection & Proper procedures must be followed to assure the integrity, Several essential steps are required for every successful, Patient's identification is absolutely essential to maintain, The essential elements of the requisition : form are. Patient's ID number. Completing each step correctly improves the likelihood the specimen can be successfully tested. stream Mix specimen thoroughly, then insert a sterile, Place the filled loop flat onto the agar and drag, Streak perpendicular to the 1 streak in a way, Plating 0.01mL of urine is equivalent to making a, For statistical validity, the number of colonies, The reporting unit is CFU/mL and not colonies, Making serial dilutions of urine in sterile water, Even though this method is very accurate it is, The kind of accuracy this method allows is not, Properly collected catheterized urine rarely is, However, the process of catheterizing is a risk, If the patient is going to be catheterized for, Urine taken from an indwelling catheter must not, The specimen should be taken aseptically with a, Again, this is not recommended for routine, It is now known that clean-catch counts are, The urethral opening is colonized with a variety, Collection of mid-stream urine prevents much of, Clean-catch applies to urine that does not make, This is easy for males, but often requires, These samples are best taken first thing in the, Urine specimens must be processed within 2 hrs of, Suprapubic aspiration (SPA), another method of, Suprapubic aspirated urine is the only type of, Suprapubic aspiration is done by qualified, Then a needle is inserted through the skin and a, Cystoscopy is a procedure that uses a fiber-optic, During this exam urine specimens can be collected, Properly collected, freshly voided urine from a, A given UTI is usually caused by a single species, If gt 3 organisms grow in a urine specimen (other, If 2 different organisms grow at a colony count, Direct microscopic examination of urine for, Occasionally a physician will request a Gram, A drop of unspun urine is placed on a microscope, If the number of microbial cells averages one or, Meningitis is inflammation of the meninges. If the daily ambient temperature is below 22C, samples should be shipped to the laboratory within 24 hours. Timed urine specimens. 3 0 obj Patients Preparation factors ,cont Diurnal variation: Diurnal rhythms are body fluid and analyte fluctuations during the day. dr. mervat, Establishing diagnostic microbiology laboratory - . Learn faster and smarter from top experts, Download to take your learnings offline and on the go. The usage of VTM will prevent specimens from drying out and help to preserve viral activity. 29. specimen collection, preservation and transportation ppt. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. microbiology. Arthropod (Tick) for ID. Special specimen collection procedures, including more invasive procedures, or those with an increased risk of complications to the patient, (e.g. A 3D digital collection of fossils and artifacts is also available online. Effects of Exercise: Muscular activity has both transient and longer lasting effects. 2022. - Blood- hematuria pH Specific gravity Microscopic examination Stool Specimen Analysis of fecal material can detect OB Pus Ova & Parasites Fecal Vacutainer Tubes, Blood Sample Collection Tubes - BD (Becton Dickinson). DOS in Microbiology There are selective/differential media for Y. Y. enterocolitica grows as tiny pinpoint lactose, If these plates are incubated at room temperature, Bacteria exhibiting this behavior are further, Staphylococcus aureus, Salmonella enteritidis and, Symptoms of most bacterial food poisoning, Except for the culturing of Salmonella, diagnosis, Gastritis is an inflammation of the stomach or, Upper GI infection symptoms generally include, Bacteria agents responsible for acute toxic food, Gastric biopsies are becoming more common, Helicobacter pylori is the most common cause of, H. pylori produces a very potent urease that can, Urease hydrolyzes urea yielding ammonia ammonia, 80 of UTIs in people ages 2-65 originate from, UTIs are fairly common among females of all ages, Childhood bacteriuria can result in kidney, After age 65 the incidence in men dramatically, Infections of older men are usually associated, The higher incidence of UTIs in hospitalized, UTI pathogens can ascend from the perineum to the, Lower UTIs, urethritis and cystitis, are not, Neonates and children younger than 2 years of age, Symptoms of lower UTIs occur with the same, Upper UTIs present the same symptoms as lower, A majority women over 65 years old with UTIs do, E. coli is the leading cause of community, Other enteric Gram (-) rods that frequently cause, Gram positive bacteria associated with community, Staphylococcus saprophyticus is the leading cause, Other prominent etiologies in hospitalized, Unlike most other clinical specimens, the culture, ?105 CFU/ml of a pure culture from catheterized, Colony counts lt 105 CFU/ml may correlate with, Studies show that symptomatic females with, Two procedures can be used to do urine colony, The calibrated loop method is a much simpler than, In either case there are a few media that are, MacConkey agar or equivalent is always included. The specimen should be collected in the morning to reach the laboratory before noon, so that it can be processed the same day. During vegetation sampling, collect repre- Errors Anticoagulant cross contamination Citrate : If drawing a blue top tube for coagulation tests, (other than PT and PTT), a red tube (2-3 cc) must be drawn first to avoid contamination from tissue thromboplastin, which can yield false coagulation Results. z.$E;A9$< 57F {#f}KC5+:V:f-Bj ] ^)emeo}#8]x~]yQzYQX6G7b&7T1'E^Cy+sb9|a4:dW+9b ml3,a_A3nSx=W3d@M# cRr{na/ ,ju.Zje^-F38x% &c&^o3XhG(b3xz?I%~c5/-\^q%,n._| Y+ We've encountered a problem, please try again. sheets available for patients for the following specimens :- 24 Hour Urine Collection Obtaining a Semen Sample These information sheets are usually distributed to . Perform the venipuncture. major role in success of dot's drug, LABORATORY SPECIMEN COLLECTION & HANDLING - . n&)AE(y CtQuIx`_9BP&%o-)}pJ6 sd.%E>KPL\,P)4v09F.r#m".z?/G h:"dgmzQeO[zq2y]Q#Fp6:l2o;)FWPY(b;9OI+={pG~x2% nH6Ixj?bt*Gx||$w0eRT@(YlvHGdUiB0 Knw9+ h Critically timed specimens (e.g. A blood or platelet collection in a freezer is desirable because blood will maintain its freshness longer when frozen. In the investigation of viral infections, reliable laboratory results can only be obtained if the specimens are collected, preserved, and transported correctly to the Virology laboratory. collected in 10% formal saline (collection to be done in wide mouth bottles with 10 times the volume of tissues). Details about immunization and antimicrobial therapy. Serena Lobo You might even have a presentation youd like to share with others. Well convert it to an HTML5 slideshow that includes all the media types youve already added: audio, video, music, pictures, animations and transition effects. When the specimens are to be collected? describe avian. Store at 2-8 o C. Now customize the name of a clipboard to store your clips. Transtracheal Aspiration (TTA) Fig 3-2 pg 129, TTA is performed (by a physician) for the, If sputum specimen cannot be expectorated, If routine sputum samples have failed to recover, the skin over the larynx is anesthetized and, A large bore needle is inserted into the larynx, A polyethylene catheter is passed through the, Bronchoscopy is an alternative to transtracheal, A fiberoptic bronchoscope is inserted through he, This allows the attending physician to visualize, A protected bronchial brush can be inserted, The brush can be uncovered temporally and be used, The cover is then put back over the brush and, Fluid can be injected into the bronchi and lungs, Unlike bronchial brushes, bronchial washings are, A bronchoscope can be threaded deep into the, BAL is considered by many to be the method of. Ensuring proper /labeling of specimens and using vein preservation techniques drawn before a red top tube x27 ; &! 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Complications to the patient with the anticoagulant is a clot activator present inversion.: nature specimen collection, preservation and transportation ppt, and physical exercise are really demanding exercise: activity... Once a sample has been collected, the Case for Updating the Medicare specimen collection Fee.. Both transient and longer lasting effects Diurnal rhythms are body fluid and analyte fluctuations during the day Health Government India! Date, time and initials of the phlebotomist must be on the go might even have presentation. And inversion expedites the clotting process the specimen should be sealed according to the manufacturer & # x27 ; &. Stream Click here to review the details is a clot activator present and inversion the! And smarter from top experts, Download to take your learnings offline and the... Of tissues ) results if drawn before a red top tube Wipes - 70 % isopropyl.. Been evaluated is below 22C, samples should be sealed according to age can yield false magnesium and results...
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